Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Apr 12;10:852561. doi: 10.3389/fcell.2022.852561

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2022 Wang, Ye, Zhou, Guo, Xiong, Gong, Jiang and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

CreER ^T2 /SynA ^−/− mouse and the phenotype of placentas and fetuses. (A) Genotyping of newborn mouse pups for the CreER ^T2 /SynA ^fl/fl genotype (1# & 4#). The expected products for Cre and SynA ^fl/fl gene were 530 bp and 367 bp in size, respectively. (B) The tamoxifen-induced group showed markedly abnormal intrauterine littermates (E17.5 d). Intrauterine littermates were arranged regularly and evenly in the uninduced group but composed of living (*) and partially/fully-absorbed (black/white arrow) littermates in the induced group. (C) Relative expression of syncytin-a gene in placentas from living, partially-absorbed and fully-absorbed embryo in the induced group compared to living embryo in the uninduced group. Placentas with partially-absorbed embryos and fully-absorbed embryos were determined as CreER ^T2 /SynA ^−/−(E17.5 d). ***: p < 0.001. (D) Proportion of littermates with different phenotypes in the induced group. The proportion of absorbed littermates increased gradually as gestation progressed from E14.5 to E18.5 d. (E) Representative embryos and placentas of CreER ^T2 /SynA ^−/− and CreER ^T2 /SynA ^fl/fl genotypes (E18.5 d). (F) H&E staining of representative embryos (E18.5 d). (i) Bone, (ii) brain, (iii) liver and (iv) skin.