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. 2022 Apr 12;16:800883. doi: 10.3389/fnins.2022.800883

FIGURE 4.

FIGURE 4

Effects of mitochondrial transplantation on cellular apoptosis in the injured spinal cord of rats with traumatic SCI. (A) Representative micrograph of Western blots of cleaved caspase-3, Bcl-2, and BAX in sham control groups. (B–D) Corresponding quantitative results of relative expression of cleaved caspase-3, Bcl-2, and BAX in sham control groups. (E) Representative micrograph of Western blots of cleaved caspase-3, Bcl-2, and BAX in SCI groups on PID 1. (F–H) Corresponding quantitative results of relative expression of cleaved caspase-3, Bcl-2, and BAX in SCI groups on PID 1. The 1-cm spinal cord specimens centered at the epicenter of the injured site were used in Western blots. (I) Representative micrographs of TUNEL assay. The white arrowheads indicate both DAPI and TUNEL dual-positive cells. The sections used in TUNEL assay were obtained from the 1-cm spinal cord specimens centered at the epicenter of the injured site. Scale = 100 μm. (J) Quantitative results of the TUNEL assay. Data were expressed as mean ± standard deviation. *p < 0.05, ***p < 0.001 two-tailed Student’s t-test. N = 3–5. MT, mitochondria transplantation.