Figure 2.

SPM receptors are expressed in mouse bladder. All panels are immunohistochemistry performed on 5 µm sections of formalin-fixed, paraffin-embedded mouse bladders from untreated control mice of ≈7–11 weeks of age, as detailed in the Methods section. The primary antibody target is indicated on each panel, and all were rabbit antibodies (see Table 1). The first panel is an isotype control using a rabbit IgG isotype control from Novus Biologicals (Centennial, CO). All parameters for the slides used in this figure (incubation times, HRP product development times, etc.) were identical between all samples and for each antibody and all slides specifically used in this figure were stained on the same day. Micrographs were taken with identical settings (exposure times, light intensity, etc.). Scale bars = 50 µm. The various layers (urothelial, interstitial, and smooth muscle) are delineated with dash lines and labeled on the micrographs.