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. 2022 Jan 20;9:uhab085. doi: 10.1093/hr/uhab085

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© The Author(s) 2022. Published by Oxford University Press on behalf of Nanjing Agricultural University

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Characteristics of the candidate gene CSESR2. A, The results of a Y1H assay showing direct binding between CsESR2 and the CsACS2 promoter. Vector combinations of pGADT7-53/p53-His2 and pGADT7/p53-His2 were used as the positive and negative controls, respectively. A, A series of 3-AT concentrations (30 mM, 50 mM, and 70 mM) were used for the test of yeast growth on selective medium (−Leu, −Trp, -His), and all combinations grew normally on non-selective medium (−Leu, −Trp). B, Identification of conserved motifs in the amino acid sequences of ESR homologs (AtESR1, AT1G12980; AtESR2, AT1G24590; SlESR2/LEAFLESS, SL05G013540; CsESR2, Csa5G598600). C, Conserved sequence analysis of motif1 (red rectangle) and motif2 (blue rectangle) in B. D, Subcellular localization analysis of CsESR2 in tobacco leaf epidermal cell. 35S:GFP was used as the positive control, and DAPI staining indicated the nucleus. Bar = 50 μm.