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. 2022 Jan 20;9:uhab085. doi: 10.1093/hr/uhab085

Figure 6.

Figure 6

CsESR2 directly regulates the expression of CsACS2. A, Schematic diagram of the vectors used in the tobacco transient expression system. B, GUS staining was used to detect the effect of CsESR2 on the promoter activity of CsACS2. C, GUS enzyme activity assay was used to quantitatively analyze the influence of CsESR2 on the activity of the CsACS2 promoter. The data are presented as the average of ten biological replicates, and error bars represent the SD. Student’s t-test was used to determine significant differences (**p<0.01). D, Schematic diagram of the vectors used in the dual luciferase reporter system. E, Luc enzyme activity assay was used to quantitatively analyze the influence of CsESR2 on the promoter activity of CsACS2. The data are presented as the average of ten biological replicates, and error bars represent the SD. Student’s t-test was used to determine significant differences (**p<0.01). F, Six promoter regions (P1 to P6) were used in the ChIP-PCR assay. The two black ellipses represent the two ERE elements in the promoter. G, ChIP-PCR assay in transgenic tobacco leaves with 35S:CsESR2-GFP-6His construct. Uninjected transgenic tobacco leaves were used as a negative control. An “input DNA” control was used as an internal reference for normalization. Error bars represent the SD of three biological replicates.