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. 2022 Apr 22;13:404–410. doi: 10.3762/bjnano.13.33

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Copyright © 2022, Li et al.

This is an open access article licensed under the terms of the Beilstein-Institut Open Access License Agreement (https://www.beilstein-journals.org/bjnano/terms/terms), which is identical to the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0). The reuse of material under this license requires that the author(s), source and license are credited. Third-party material in this article could be subject to other licenses (typically indicated in the credit line), and in this case, users are required to obtain permission from the license holder to reuse the material.

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Elastic modulus and water content of specimens in different treatment groups. (a) The elastic moduli of fresh (n = 5), frozen (n = 5), desiccated (n = 5), and rehydrated (n = 5) hind tibiae are 4.8 ± 0.2, 7.3 ± 0.2, 9.4 ± 0.5, and 6.2 ± 0.2 GPa, respectively. Significant differences were determined via Holm–Sidak one-way ANOVA. Different capital letters indicate significant differences (P < 0.001). (b) Elastic modulus of fresh tibiae as a function of the recorded time. Different samples are presented in different colors. Each data point represents the elastic modulus obtained from a distinct indentation site. (c) Water contents of fresh (n = 15), frozen (n = 15), desiccated (n = 15), and rehydrated (n = 15) hind tibiae. Significant differences were determined via Kruskal–Wallis one-way ANOVA on ranks. Different capital letters indicate significant differences (P < 0.05). (d) Water contents of fresh (n = 3) and frozen tibiae (n = 3) as function of the time.