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. 2022 Apr 12;10:881544. doi: 10.3389/fbioe.2022.881544

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Copyright © 2022 Fu, Li, Xue, Yu, Yang, Tao, Li, Wang, Zhang and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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In vitro functions of NSMRH in MM-231 cells. (A) Effects of different conditions on MM-231 cells viability at 48 h. (B) Edu staining of MM-231 cells, red fluorescence represents proliferating cells, scale bars: 100 μm. (C) A clonogenic assay was performed. Colonies larger than 50 cells were counted, and phase-contrast images were taken, scale bar: 2.5 mm. (D) Survival curves of MM-231 cells exposed to increased irradiation. (E) Effect of NSMRH on MM-231 cells migration. Representative images showing tumor cells migration, scale bars: 100 μm. (F) The figure shows quantitative analysis of a wound healing assay, with triplicate measurements of three independent experiments. (G) Photomicrographs illustrate representative fields of invaded cells, scale bars: 100 μm. (H) The relative number of invaded cells was calculated, and the data are presented in a histogram from three independent experiments.