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Confirmation of mutation in TaIPK1.A gene by sequencing. TaIPK1.A mutant alleles detected in To wheat plants. Insertions and deletions are represented by magenta font or magenta hyphens respectively. For each line (L4M1_A to L13M3_F), 12 clones were examined and the frequencies of each mutant allele are represented at the right side of the panel (Additional file 4).
