Fig. 5.

Micronutrient and phenotypic analysis of the mature T₂ grains of genome edited mutant lines. (A) Total Phytic acid (PA) in the mature T₂ seeds of genome edited (GE) mutant lines. PA was measured in the mature T₂ seeds from the primary tiller of each GE mutant line. (B, C) Fe and Zn concentration (μg/g) was estimated by using Atomic absorption spectrometry (AAS). (D) Seed weight of control (CB-16) and GE mutant lines. (E) Seed length of control (CB-16) and GE mutant lines. (F) Seed width of control (CB-16) and GE mutant lines. (G) Representative picture of spikes of wheat GE mutant lines and control (scale bar = 1.5 cm). (H) Spike length (cm) and spikelet count from the primary tiller of GE mutant lines and control CB16 at T₂ stage. The data indicates the mean ± SE of six biological replicates. Student’s t-test was used to compare the significant variation between each transgenic line and control. *,** and *** shows statistical significance at P < 0.05, P < 0.01 and P < 0.001, respectively, and ns = non-significant.