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. 2022 Apr 12;12:844477. doi: 10.3389/fonc.2022.844477

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Copyright © 2022 Ning, Sun, Su, Tan, Tang, Sayed, Li, Xue, Liu, Chen and Lu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The CK1δ/CK1ϵ/β-catenin/Tip60 complex is detected in colon cancer cells and β-catenin interacts with Tip60 in a CK1δ/CK1ϵ-dependent manner. (A, B) Cell lysates from SW480 (A) or HCT116 (B) cells were immunoprecipitated with normal IgG control or anti-β-catenin agarose beads, Immunoblot analysis was performed to detect the interaction among β-catenin, Tip60 and CK1δ/ϵ using the indicated antibodies. (C, D) SW480 (C) or HCT116 (D) cells were infected with shNC, shCK1δ-2, shCK1ϵ-2, shCK1δ/ϵ-2 lentivirus, then cells were lysed and subjected to immunoprecipitation with anti-β-catenin agarose beads. Immunoblotting was performed using the indicated antibodies.