Skip to main content
. 2021 Jul 10;37:107–117. doi: 10.1016/j.jare.2021.07.004

Fig. 6.

Fig. 6

Autophagy inhibition/activation and the effect of LRRK2 on cardiomyocytes. a-d: NRCMs were transfected with LRRK2 siRNA and treated with the autophagy inhibitors 3-MA, bafilomycin A1 and chloroquine. a. P62 level in the indicated group (n = 6). b. α-Actin staining (n = 5). c. Cell surface area in PE-stimulated cells (n = 50). d. mRNA levels of ANP and β-MHC in cells (n = 6). *P < 0.05 vs. siRNA; # P < 0.05 vs. PE; εP < 0.05 vs. PE + siLRRK2.e-g: NRCMs were transfected with Ad-LRRK2 and treated with the autophagy activator rapamycin. d. α-Actin staining (n = 5). e. Cell surface area in PE-stimulated cells (n = 50). f. mRNA levels of ANP and β-MHC in cells (n = 6). *P < 0.05 vs. Ad-NC; # P < 0.05 vs. PE; εP < 0.05 vs. PE + Ad-LRRK2.