Fig. 1.

DS is associated with the upregulation of CBS and 3-MST enzymes in a brain-region-specific manner. Following the cognitive assessment, animals were euthanized and tissue from the prefrontal cortex (PFC), hippocampus (HIP), entorhinal cortex (ETC), and the basal forebrain (BF) brain regions were collected for protein extraction. Protein samples were processed for immunoblotting detection of the H₂S producing enzymes cystathionine β-synthase (CBS) (A, B, C), 3-mercaptopyruvate sulfurtransferase (3MST) (A, D), and cystathionine γ-lyase (CSE) (A, E). (B): Quantification of the proteolytic, 45 kDa isoform of CBS. The expression of β-actin served as loading control for our analysis. Data, expressed as mean ± SEM of 5 animals per experimental condition, were analyzed by two-way ANOVA followed by post hoc Bonferroni multiple comparison t-tests at each brain region of interest. *p≤0.05 or **p≤0.01 shows significant differences between saline-treated DS rats and saline-treated WT rats; ^#p ≤ 0.05 or ^##p ≤ 0.01 shows significant effect of AOAA treatment on the expression of various H₂S-producing enzymes in Dup(Rno20)Yah rats.