Fig. 2.
DS differentially modulates the expression of various expression enzymes involved in H2S degradation in a brain-region-specific manner. Following the cognitive assessment, animals were euthanized and tissue from the prefrontal cortex (PFC), hippocampus (HIP), entorhinal cortex (ETC), and the basal forebrain (BF) brain regions were collected for protein extraction. Protein samples were processed for immunoblotting detection of the H2S catabolizing enzymes (A, B) ethylmalonic encephalopathy 1 protein (ETHE1), (A, C) rhodanese/thiosulfate sulfurtransferase (TST), and (D) sulfide quinone reductase (SQR). The expression of β-actin served as loading control for our analysis. Data, expressed as mean ± SEM of 5 animals per experimental condition, were analyzed by two-way ANOVA followed by post hoc Bonferroni multiple comparison t-tests at each brain region of interest. *p≤0.05 shows significant differences between saline-treated DS rats and saline-treated WT rats; #p ≤ 0.05 shows significant effect of DS or AOAA treatment on the expression of various H2S-regulating enzymes in Dup(Rno20)Yah rats.