Fig. 3.

CBS expression in the DS brain is localized in astrocytes and vascular tissues surrounded by astrocytic end-feet. Immunohistochemical staining of DS rat brain sections (40 μm thick) was analyzed in 4 different brain regions, including the prefrontal cortex (PFC), the basal forebrain (BF), the hippocampus (HIP), and the entorhinal cortex (ETC). Coronal brain sections were first scanned using the NanoZoomer in brightfield mode at small magnification in single layer (A). The regions of interest within different brain sections (black squares) were selected according to Paxinos-Franklin atlas and scanned using the NanoZoomer in fluorescent mode at 15X magnification (B), and confocal microscope at 40X magnification (C). Confocal images are shown as maximum intensity projection of the whole z-stack (40 μm), with z-step equal 0,42 μm. Scale bars represent 2 mm (A), 200 μm (B), and 60 μm (C), respectively. Immunofluorescence labeling for CBS (red), GFAP (green) and counterstaining with DAPI (blue) is shown. Identical acquisition settings were applied between different brain regions and groups. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)