Fig. 8.

Neither the genetic overdosage of CBS nor the pharmacological inhibition of CBS enzyme activity affects spontaneous alteration in vivo. (A): Schematic representation of the forced T-maze task. During the acquisition trial, the tested rats were forced to select and explore the left arm of the apparatus. After a 5-min delay, each testing subject was placed into the starting arm and allowed to explore the maze with both left and right arms accessible. Every entry of the rat to the unfamiliar, right arm of the apparatus (annotated with the "+" symbol) represented a spontaneous alteration event and counted as a success. (B): The latency till the first successful entry along with the number of successful entries to the novel arm during the retention trial was monitored. (C): Success Rate was further calculated based on the allotted time to the novel arm over the total time in both arms engaged by each testing subject. Each dot plot represents one animal. Success Rate data were expressed as mean ± SEM of 11 rats per experimental condition and plotted as bar graphs. No significant differences were noted between any of the experimental groups studied.