TABLE 2.

Reference dataset of functionally verified polyesterases having activity on PET or PET model substrates, which was used to conduct BLASTP protein homology searches against the B129SM11 annotated genome and for further phylogenetic inferences.

Enzyme	Source	Uniprot accession	References
TfH	Thermobifida fusca DSM43793	Q6A0I4	Müller et al., 2005
Tfu_0882	Thermobifida fusca YX	Q47RJ7	Chen et al., 2008
Tfu_0883	Thermobifida fusca YX	Q47RJ6	Chen et al., 2008
TfCut1	Thermobifida fusca KW3	E5BBQ2	Herrero Acero et al., 2011
TfCut2	Thermobifida fusca KW3	E5BBQ3	Herrero Acero et al., 2011
Est1	Thermobifida alba AHK119	D4Q9N1	Thumarat et al., 2015
Est119	Thermobifida alba AHK119	F7IX06	Hu et al., 2010
Thc_Cut1	Thermobifida cellulosilytica DSM44535	E9LVH8	Herrero Acero et al., 2011
Thc_Cut2	Thermobifida cellulosilytica DSM44535	E9LVH9	Herrero Acero et al., 2011
Thf42_Cut1	Thermobifida fusca DSM44342	E9LVI0	Herrero Acero et al., 2011
Tha_Cut1	Thermobifida alba DSM43185	E9LVH7	Ribitsch et al., 2012a
Thh_Est	Thermobifida halotolerans DSM44931	H6WX58	Ribitsch et al., 2012b
LCC	Metagenome from leaf-branch compost	G9BY57	Sulaiman et al., 2012
Tcur1278	Thermonospora curvata DSM43183	D1A9G5	Wei et al., 2014
Tcur0390	Thermonospora curvata DSM43183	D1A2H1	Wei et al., 2014
Cut190	Saccharomonospora viridis AHK190	W0TJ64	Kawai et al., 2014
IsPETase	Ideonella sakaiensis strain 201-F6	A0A0K8P6T7	Yoshida et al., 2016
BhrPETase	Thermophilic bacterium strain HR29	A0A2H5Z9R5	Xi et al., 2021
SM14est	Streptomyces sp. SM14 (marine)	DAC80635.1 (Genbank)	Almeida et al., 2019
PE-H	Pseudomonas aestusnigri (marine)	A0A1H6AD45	Bollinger et al., 2020
BsEstB	Bacillus subtilis 4P3-11	D7R6G8	Ribitsch et al., 2011
PET12	Polyangium brachysporum	A0A0G3BI90	Danso et al., 2018
PET2	Uncultured bacterium (marine metagenome)	C3RYL0	Danso et al., 2018
PET5	Oleispira antarctica RB-8	R4YKL9	Danso et al., 2018
PET6	Vibrio gazogenes	A0A1Z2SIQ1	Danso et al., 2018
HiC	Humicola insolens	A0A075B5G4	Ronkvist et al., 2009