Figure 4.

Adoptive transfer of SpNK or ThNK donor cells into immunodeficient hosts reveals alternative functional effects on digit tip regeneration in vivo

(A) SpNK and ThNK cells were harvested from C57Bl6/J (B6) mice, enriched, and injected into NSG mice via ACT.

(B) Representative trichrome histological characterization of SpNK and ThNK cell-treated NSG animals over the early time course of regeneration relative to vehicle and B6 animal controls (n = 4). Key regenerative stages are described in the arrow banner below.

(C) Representative μCT images of digit tip regeneration after SpNK or ThNK cell ACT.

(D) Volumetric analysis of μCT cohorts. BVR and SVR were quantification based on μCT relative to unamputated control digits (^∗∗∗p < 0.001, ^∗∗∗∗p < 0.0001; two-way ANOVA and Bonferroni ±SEM; n = 5 mice/condition).

(E) Rate of change of hard and soft tissue volume, calculated by taking the first derivative of the change in tissue volume.