Figure 2.

Directed differentiation of human iPSC lines with FUS P525L mutations and isogenic controls into MIGs

(A) Schematic of differentiation protocol of human iPSCs into MIGs.

(B) Phase-contrast images of iPSC-derived MIGs of different FUS genotypes. For time-lapse movies depicting motile pseudopodia and putative phagosomes, see Videos S1A and S1B.

(C) Flow cytometry dot plots of characteristic microglial surface markers in MIGs. FMO, fluorescence minus 1 control using WT cells.

(D) Immunocytochemistry of FUS and microglial protein TREM2 in MIGs. Scale bar, 50 μm. Presented here are clones from the AG line.