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. 2022 Feb 3;17(3):569–583. doi: 10.1016/j.stemcr.2022.01.005

Figure 3.

Figure 3

The effect of VEGF stimulation on ECM-guided endothelial differentiation

(A) Representative immunofluorescence images showing CD31 staining (red) and DAPI (blue) following differentiation on Col I or LN411 when either PBS (control) or VEGF was incubated with the ECM prior to cell seeding. C, collagen I; 411, laminin 411; VEGF, vascular endothelial growth factor.

(B) CD31 area per DAPI area following differentiation on Col I or LN411 with or without preincubation with VEGF. All conditions were normalized to the Col I control. n ≥ 5 wells per condition, which includes at least three independent experimental replicates per condition. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001.

(C) Percentage VEGF absorbed compared with VEGF added when incubated with Col I or LN411, determined by ELISA. n = 4 for all conditions. No significant differences were observed.