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. 2022 Feb 10;17(3):664–677. doi: 10.1016/j.stemcr.2022.01.007

Figure 6.

Figure 6

Inhibition of S1PR2 impairs OE regeneration and functional recovery of the olfactory system after injury

(A) H&E staining of the OE obtained from control- and JTE013-treated 2-month-old C57BL/6 mice at 0, 5, and 14 dpl.

(B) Quantitative analysis of the thickness of OE as shown in (A) (n = 7 sections from 4 mice per group).

(C) Percentage of mice that found cookies over total observed control-treated (n = 8 mice) or JTE013-treated 2-month-old C57BL/6 mice (n = 7 mice) in the buried pellet test at 14 dpl (Student’s t test).

(D) Quantitative analysis of the time to locate food in control-treated (n = 8 mice) or JTE013-treated 2-month-old C57BL/6 mice (n = 7 mice) at 14 dpl in the buried pellet test (Student’s t test).

(E and F) Double immunostaining of Ki67 (green) and p63 (red) (E) or PH3 (green) and K5 (red) (F) in the OE of control- and JTE013-treated 2-month-old C57BL/6 mice at 0, 3, and 5 dpl.

(G and H) Quantitative analysis of the percentage of both Ki67+ and p63+ cells (G) or PH3+ and K5+ (H) cells over p63+ or K5+ HBCs as shown in (E) and (F) (n = 10 sections from 4 mice per group).

(I) Double immunostaining of GAP43 (green) and OMP (red) in the OE of control- and JTE013-treated 2-month-old C57BL/6 mice at 0, 7, and 14 dpl.

(J and K) Quantitative analysis of the density of GAP43+ (J) or OMP+ (K) cells at 0, 3, 7, and 14 dpl (n = 6 sections from 4 mice per group).

Images of selected regions are shown at higher magnification. Data are mean ± SEM, two-way ANOVA with Bonferroni’s post tests unless otherwise indicated, compared with control group; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. Scale bars, 20 μm.