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. 2022 Feb 10;17(3):664–677. doi: 10.1016/j.stemcr.2022.01.007

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© 2022 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Activation of YAP signaling by XMU-MP-1 promotes OE regeneration and functional recovery of the olfactory system after injury

(A) Immunohistochemistry detected the expression of YAP in the OE of control- and XMU-MP-1-treated 2-month-old C57BL/6 mice at 0, 3, and 7 dpl.

(B) Quantitative analysis of the relative YAP density as shown in (A) (n = 6 sections from 6 mice per group).

(C) Western blot detected the expression of p-MOB1, MOB1, p-YAP, and YAP in OE of control- and XMU-MP-1-treated 2-month-old C57BL/6 mice at 7 dpl.

(D and F) Quantitative analysis of the relative p-MOB1/MOB1 (D), p-YAP/YAP (E), and YAP (F) levels as shown in (C) (normalized with control group, n = 8 blots from 4 mice per group, Student’s t test).

(G) H&E staining of the OE obtained from control- and XMU-MP-1-treated 2-month-old C57BL/6 mice at 0, 3, and 7 dpl.

(H) Quantitative analysis of the thickness of OE as shown in (G) (n = 12 sections from 6 mice per group).

(I) Quantitative analysis of the time to locate food of control-treated (n = 10 mice) and XMU-MP-1-treated 2-month-old C57BL/6 mice (n = 13 mice) in the buried pellet test at 7 dpl (Student’s t test).

(J and K) Double immunostaining of Ki67 (green) and p63 (red) (J) or PH3 (green) and K5 (red) (K) in the OE of control- and XMU-MP-1-treated 2-month-old C57BL/6 mice at 0 and 3 dpl.

(L and M) Quantitative analysis of the percentage of both Ki67⁺ and p63⁺ (L) or PH3⁺ and K5⁺ (M) cells over p63⁺ or K5⁺ HBCs as shown in (J) and (K) (n = 13 sections from 6 mice per group).

(N) Immunostaining of GAP43 (green) in the OE of control- and XMU-MP-1-treated 2-month-old C57BL/6 mice at 7 dpl.

(O) Quantitative analysis of the density of GAP43⁺ cells as shown in (N) (n = 12 sections from 6 mice per group, Student’s t test).

Images of selected regions are shown at higher magnification. Data are mean ± SEM, two-way ANOVA with Bonferroni’s post tests unless otherwise indicated, compared with control group; ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001. Scale bars, 20 μm.