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. 2022 Apr 21:10.2144/btn-2022-0018. doi: 10.2144/btn-2022-0018

Figure 3. Duplex RT-qPCR analysis of virus yield from different SARS-CoV-2 variants of concern.

Figure 3.

Vero E6 cells were exposed to a fixed virus stock dilution (10×) of different variants of concern of SARS-CoV-2 (that correlates with the following MOI: Wuhan = 3.4; 20A.EU2 = 7.4; Alpha = 1.6; Beta = 15; Delta = 0.016; Omicron = 1.6). After a 2 h incubation, supernatant (with virus) was then removed, and cells were washed in PBS and given fresh medium. At day 3 postinfection, supernatant (that contained newly produced virus particles) was collected and a duplex RT-qPCR was performed to quantify the copy numbers of the E gene (left panel) and the N gene (right panel). For each condition, two biological replicates were included, and for the RT-qPCR quantification two technical replicates of each sample were analyzed to calculate the average value. Bars represent mean ± standard deviation (SD) (n = 2).