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. 2022 Apr 21:10.2144/btn-2022-0018. doi: 10.2144/btn-2022-0018

Figure 5. Viral N staining of Vero E6 cells infected with different variants of SARS-CoV-2.

Figure 5.

Vero E6 cells were exposed to a virus stock dilution (10x, 100x or 1000x) of different variants of concern of SARS-CoV-2 (the 10x dilution correlates with the following MOI: Wuhan = 3.4; 20A.EU2 = 7.4; Alpha = 1.6; Beta = 15; Delta = 0.016; Omicron = 1.6). At day 2 post-infection, cells were collected, fixed, permeabilized and stained with an antibody that binds to the viral N protein. Dot plots show N expression in noninfected (lower left quadrant) and infected (lower right quadrant) cells from a representative experiment with multiple individual samples. Single cell analysis was done on numerous independent cells, and the individual plots shown in the figure are based on the analysis of 8,000 – 10,000 Vero E6 cells by flow cytometry. The numbers in each quadrant refer to the distribution of the cells (i.e., percentage of total cell population). The dot plot in black (left panel) represents the non-infected cell control.

FSC: Forward scatter; MFI: Mean fluorescence intensity.