Fig. 4.

Immunofluorescence analysis for tight junction proteins. (A) Claudin-1 (red); (B) Occludin (red); (C) Mean fluorescence intensity of Claudin-1; (D) Mean fluorescence intensity of occludin; Nuclei were stained with DAPI (blue). Fluorescence images were captured by ortho-fluorescent microscopy (n = 5). Data are expressed as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 were compared with the DSS group. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)