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. Author manuscript; available in PMC: 2022 Apr 26.
Published in final edited form as: Cell Metab. 2022 Mar 18;34(4):533–548.e12. doi: 10.1016/j.cmet.2022.02.016

Figure 6. Macrophages control the thermogenic capacity of BAT.

Figure 6.

(A) Flow cytometry density plot and quantitation of CD169+ bMACs. Student’s t test, *p < 0.05, n = 7 mice/group.

(B) Experimental design and t-SNE map of antigen redistribution in BAT of WT and CD169DTR mice 4 days after DT injection.

(C) Immunoblots of BAT from WT and CD169DTR mice 14 days after DT injection. HSL was used as loading control (n = 4 mice/group).

(D) Experimental design and representative fluorescence micrographs of BAT stained with the CD68 antibody (red) to detect bMACs. Quantification of CD68+ bMACs is reported (right panel). ANOVA, *p < 0.05, **p < 0.01, n = 4 mice/group.

(E–G) qPCR (E), immunoblot (F) of BAT and rectal temperature (G) of mice at 30°C and 4°C. Mice were pre-treated with liposome clodronate (CLDNT) prior to cold exposure. ANOVA, *p < 0.05, **p < 0.01, ****p < 0.001; n = 3 or n = 6 mice/group.

(H and I) Flow-cytometry quantitation of total EVs (H) and MTG+ EVs (I) released from BAT. Student’s t test, *p < 0.05, **p < 0.01; n = 3 mice/group.

(J) Representative iunoblots of BAT EVs. Ponceau was used as loading control (n = 3 mice/group). See also Figure S6.