Table 1.
List of IGF-1 peptides and SRM transitions monitored with their m/z values and fragment ions. Sequences chosen respective as qualifier transition and quantifier biomarker are in bold.
| Peptide sequence | Precursor ion [M + 2H]2+m/z | Product ion m/z | Fragment ion |
|---|---|---|---|
| GFYFNKPTGYGSSSR | 834.4 | 1153.6 | y11 |
| GFYFNKPTGYGSSSR (selected qualifier transition) | 834.4 | 1039.5 | y10 |
| GFYFNKPTGYGSSSR | 834.4 | 911.4 | y9 |
| GFYFNKPTGYGSSSR | 834.4 | 757.4 | b6 |
| GFYFNKPTGYGSSSR | 834.4 | 854.4 | b7 |
| GFYFNKPTGYGSSSR | 834.4 | 955.5 | b8 |
| GFYFNKPTGYGSSSR | 834.4 | 1012.5 | b9 |
| APQTGIVDEC[+57]C[+57]FR | 776.8 | 1481.6 | y12 |
| APQTGIVDEC[+57]C[+57]FR | 776.8 | 1098.5 | y8 |
| APQTGIVDEC[+57]C[+57]FR | 776.8 | 985.8 | y7 |
| APQTGIVDEC[+57]C[+57]FR | 776.8 | 886.3 | y6 |
| APQTGIVDEC[+57]C[+57]FR | 776.8 | 782.4 | b8 |
| APQTGIVDEC[+57]C[+57]FR | 776.8 | 911.4 | b9 |
| APQTGIVDEC[+57]C[+57]FR | 776.8 | 1071.5 | b10 |
| LEMYC[+57]APLKPAK | 710.9 | 1047.6 | y9 |
| LEMYC[+57]APLKPAK | 710.9 | 884.5 | y8 |
| LEMYC[+57]APLKPAK | 710.9 | 724.5 | y7 |
| LEMYC[+57]APLKPAK | 710.9 | 653.4 | y6 |
| LEMYC[+57]APLKPAK (selected quantifier biomarker) | 710.9 | 315.2 | y3 |
| LEMYC[+57]APLKPAK | 710.9 | 768.3 | b6 |
| LEMYC[+57]APLKPAK | 710.9 | 865.4 | b7 |
| LEMYC[+57]APLKPAK | 710.9 | 978.4 | b8 |
| LEMYC[+57]APLKPAK | 710.9 | 1106.5 | b9 |