Table 2.
PLE Extract |
AChE |
BChE |
LOX |
ABTS |
ROS - ORAC |
RNS |
Yield |
||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
(IC 50 µg/mL) | % | ||||||||||||||||||||||||||
Temp (°C) | Time (min) | ||||||||||||||||||||||||||
100 | 10 | 181.3c | ± | 22.7 | 160.1abcd | ± | 9.2 | 75.3f | ± | 8.7 | 16.5e | ± | 0.4 | 4.0ef | ± | 0.4 | 872c | ± | 16 | 1.5cde | ± | 0.1 | |||||
62.5 | 30 | 133.0de | ± | 12.0 | 143.0cde | ± | 11.4 | 95.7def | ± | 6.8 | 24.0 cd | ± | 1.8 | 4.4def | ± | 0.2 | 480f | ± | 55 | 1.1def | ± | 0.1 | |||||
62.5 | 10 | 113.6e | ± | 9.7 | 139.9de | ± | 9.9 | 115.4 cd | ± | 12.9 | 27.5c | ± | 1.6 | 4.6de | ± | 0.1 | 671de | ± | 48 | 0.7df | ± | 0.1 | |||||
62.5* | 20* | 117.9de | ± | 12.6 | 127.6ef | ± | 9.1 | 101.1de | ± | 4.5 | 23.1cde | ± | 2.7 | 4.8d | ± | 0.4 | 453.3f | ± | 57 | 0.7df | ± | 0.1 | |||||
25 | 30 | 298.2a | ± | 3.2 | 190.2a | ± | 15.9 | 161.4a | ± | 7.4 | 72.9ab | ± | 3.1 | 7.3a | ± | 0.4 | 1060ab | ± | 103 | 0.4f | ± | 0.0 | |||||
100# | 30# | 137.1de | ± | 8.1 | 147.0cde | ± | 7.5 | 76.1f | ± | 10.4 | 13.5e | ± | 0.8 | 4.4def | ± | 0.4 | 1199a | ± | 98 | 2.1b | ± | 0.2 | |||||
100 | 20 | 150.0 cd | ± | 11.2 | 153.9bcde | ± | 15.8 | 78.0f | ± | 5.5 | 18.2cde | ± | 0.6 | 3.9f | ± | 0.3 | 841 cd | ± | 99 | 1.9 cd | ± | 0.1 | |||||
25 | 20 | 218.4b | ± | 21.4 | 181.9ab | ± | 23.7 | 152.6ab | ± | 12.9 | 71.4b | ± | 7.1 | 6.6b | ± | 0.2 | 877bc | ± | 119 | 0.4f | ± | 0.0 | |||||
25 | 10 | 225.0b | ± | 8.1 | 171.9abc | ± | 5.4 | 125.4c | ± | 9.8 | 75.5ab | ± | 7.4 | 6.0bc | ± | 0.0 | 1073a | ± | 91 | 0.4f | ± | 0.0 | |||||
120 | 30 | 80.1ef | ± | 8.1 | 2.8bc | ± | 0.4 | ||||||||||||||||||||
160 | 30 | 89.0ef | ± | 0.70 | 9.2a | ± | 1.0 | ||||||||||||||||||||
Conventional | 179.2c | ± | 25.1 | 102.2f | ± | 4.5 | 130.7c | ± | 7.0 | 84.1a | ± | 7.7 | 5.5c | ± | 0.1 | 556.9ef | ± | 11 | 0.5f | ± | 0.0 | ||||||
Positive control | |||||||||||||||||||||||||||
Galantamine | 0.8f | ± | 0.0 | 2.5 g | ± | 0.0 | |||||||||||||||||||||
Quercetin | 137.2bc | ± | 15.4 | ||||||||||||||||||||||||
Ascorbic acid | 23.4cde | ± | 1.1 | 2.3 g | ± | 0.1 | 1100a | ± | 13 | ||||||||||||||||||
Different letters in the same column show statistically differences (p < 0.05).
* Central point.
# Optimal point.