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. 2022 Feb 4;13:100242. doi: 10.1016/j.fochx.2022.100242

Table 2.

Yield in % and IC 50 values from in to vitro assays of different orange juice by-products extracts using AChE, BChE, LOX, ABTS, ROS and RNS assays.

PLE Extract AChE

BChE

LOX

ABTS

ROS - ORAC

RNS
Yield
(IC 50 µg/mL) %
Temp (°C) Time (min)
100 10 181.3c ± 22.7 160.1abcd ± 9.2 75.3f ± 8.7 16.5e ± 0.4 4.0ef ± 0.4 872c ± 16 1.5cde ± 0.1
62.5 30 133.0de ± 12.0 143.0cde ± 11.4 95.7def ± 6.8 24.0 cd ± 1.8 4.4def ± 0.2 480f ± 55 1.1def ± 0.1
62.5 10 113.6e ± 9.7 139.9de ± 9.9 115.4 cd ± 12.9 27.5c ± 1.6 4.6de ± 0.1 671de ± 48 0.7df ± 0.1
62.5* 20* 117.9de ± 12.6 127.6ef ± 9.1 101.1de ± 4.5 23.1cde ± 2.7 4.8d ± 0.4 453.3f ± 57 0.7df ± 0.1
25 30 298.2a ± 3.2 190.2a ± 15.9 161.4a ± 7.4 72.9ab ± 3.1 7.3a ± 0.4 1060ab ± 103 0.4f ± 0.0
100# 30# 137.1de ± 8.1 147.0cde ± 7.5 76.1f ± 10.4 13.5e ± 0.8 4.4def ± 0.4 1199a ± 98 2.1b ± 0.2
100 20 150.0 cd ± 11.2 153.9bcde ± 15.8 78.0f ± 5.5 18.2cde ± 0.6 3.9f ± 0.3 841 cd ± 99 1.9 cd ± 0.1
25 20 218.4b ± 21.4 181.9ab ± 23.7 152.6ab ± 12.9 71.4b ± 7.1 6.6b ± 0.2 877bc ± 119 0.4f ± 0.0
25 10 225.0b ± 8.1 171.9abc ± 5.4 125.4c ± 9.8 75.5ab ± 7.4 6.0bc ± 0.0 1073a ± 91 0.4f ± 0.0
120 30 80.1ef ± 8.1 2.8bc ± 0.4
160 30 89.0ef ± 0.70 9.2a ± 1.0
Conventional 179.2c ± 25.1 102.2f ± 4.5 130.7c ± 7.0 84.1a ± 7.7 5.5c ± 0.1 556.9ef ± 11 0.5f ± 0.0
Positive control
Galantamine 0.8f ± 0.0 2.5 g ± 0.0
Quercetin 137.2bc ± 15.4
Ascorbic acid 23.4cde ± 1.1 2.3 g ± 0.1 1100a ± 13

Different letters in the same column show statistically differences (p < 0.05).

* Central point.

# Optimal point.