Figure 1.
The construction of standard testing sets and the strategy of human proteome quantitation for PDX model. Lysates of AGS cells and MEF cells were mixed at certain ratios to form four standard testing sets. Mixtures were digested by trypsin and fractionated via house-packed C18 tip, followed with LC–MS/MS analysis. Data were searched against the human protein database or HM database for sequential evaluation. MEF, mouse fibroblast; LC–MS/MS, liquid chromatography coupled to tandem mass spectrometry; HM, human–mouse combined; HU, human-unique; SH, human–mouse shared.