Fig. 5.

Immunohistochemical localization of A. pavonina trypsin inhibitors. Paraffin-embedded of A. pavonina seed was incubated with soybean trypsin inhibitor antibodies, followed by horseradish peroxidase–labeled polymer conjugated to anti-rabbit antibodies (DAKO). Bright-field view of hematoxylin-stained section of A. pavonina seed (Fig. 5A). Brown staining shows the location of specifically bound antibodies in the seed tissue (Fig. 5B, arrow). Immunofluorescence scanning confocal microscopy. Paraffin-embedded of A. pavonina seed was incubated with soybean trypsin inhibitor antibodies and goat anti-rabbit Alexa Fluor Plus 488 and observed by scanning confocal microscopy. Note green fluorescence signals mostly around the peripheral region of the cells (Fig. 5C, arrow). An overlay of bright-field (Fig. 5D) and fluorescence image (Fig. 5C) clearly shows the localization of trypsin inhibitors mostly in the cytosol of the cells (Fig. 5E, arow). PSV, protein storage vacuole. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)