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. 2021 Oct 15;2:218–228. doi: 10.1016/j.crimmu.2021.10.004

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© 2021 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. s4 — Gene ontology (GO) enrichment analysis of proteins detected in plasma membrane (PM)-enriched and intracellular compartment (IC)-enriched microsome fractions of splenic cDCs and B cells purified from WT, Marchf1^-/-, or Marchf8^-/- mice. PM fractions were purified from post-nuclear supernatants of mAb surface stained cDCs and B cells via magnetic immunoaffinity. IC (intracellular compartments) was retrieved from the post-nuclear supernatant of homogenized cells following PM fraction extraction. IC and PM fraction were analyzed using semi-quantitative mass spectrometry and GO term enrichment analysis was performed using the Bioconductor clusterProfiler package (Yu et al., 2012) with GO-IDs grouped based on experimentally verified Gene Ontology (GO) annotations.