Fig. 1.

Ubiquitination of MHC II and CD86 by MARCH1 and MARCH8 in haemopoietic professional APCs. Surface expression levels of MHC II and CD86 in (A) splenic cDC1s, cDC2s, pDCs, B cells, macrophages (MAC), and CD4⁺/CD8⁺ T cells, (B) blood B cells, (C) thymic cDC1s, cDC2s, and B cells, (D) peritoneal cDC1s, cDC2s, B cells, and small/large MAC, and (E) lung cDC1s, cDC2s, pDC, B cells, and alveolar/interstitial MAC, all purified from WT mice or mice deficient in either MARCH1 or MARCH8. Bars represent mean ​± ​SD with each symbol representing an individual mouse (n ​= ​4–5). Statistical analysis was performed using one-way ANOVA followed by Sidak's multiple comparisons test. ∗∗∗∗p ​< ​0.0001, ∗∗∗p ​< ​0.0002, ∗∗p ​< ​0.002, ∗p ​< ​0.03, n.s. not significant.