Fig. 6.

Ubiquitination of MHC II, CD86 and putative substrates by MARCH1 and MARCH8 in non-haemopoietic APCs. (A) Surface expression of MHC II, CD86, CD80, CD40, CD44, CD95, and CD98 in medullary and cortical thymic epithelial cells (mTECs and cTECs) purified from WT, Marchf1^-/- and Marchf8^-/- mice. (B) Surface expression of MHC II and CD86 in endothelial cells, type I and type II alveolar epithelial cells (AECs) as well as bronchiolar epithelial cells, purified from the lung of WT, Marchf1^-/- and Marchf8^-/- mice. In all cases a fluorescence-minus-one (FMO) control was included, for which cells were incubated with the corresponding multi-colour staining panel, excluding the fluorescently labelled antibody species of interest. Bars represent mean ​± ​SD with each symbol representing an individual mouse (n ​= ​5). Statistical analysis was performed using one-way ANOVA followed by Sidak's multiple comparisons test. ∗∗∗∗p ​< ​0.0001, ∗∗∗p ​< ​0.0002, n.s. not significant.