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. 2021 Sep 22;37:169–184. doi: 10.1016/j.jare.2021.09.009

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© 2022 The Authors. Published by Elsevier B.V. on behalf of Cairo University.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 2 — miR-532-5p inhibits CC cell metastasis and lymphangiogenesis via EMT and reduces intracellular LD accumulation and metabolic reprogramming. (A) miR-532-5p mimics significantly decreased cell proliferation compared with that of miR-Ctrl cells, as indicated by the MTT assay. (B) The effects of miR-532-5p on the cell invasion abilities (scale bars, 500 µm). (C) D2-40 staining for lymph vessel in CC tissues (representative image, n = 10, 200×). (D) The effects of miR-532-5p on the tube formation of HLECs (scale bars, 100 µm). (E) The protein levels of EMT markers. (F) The LDs staining with BODIPY 493/503 (Scale bars, 25 µm). (G) The cellular content of PLs and TGs. (H) The protein levels of key lipid metabolic enzymes. *, P < 0.05; **, P < 0.01; ***, P < 0.001.