SCFAs increase survival in TNBS-exposed larvae. A. Experimental design of SCFAs and TNBS treatment. Larvae were treated with SCFAs from 3 to 10 dpf with a change of medium at day 7. TNBS was added to the medium at 7 dpf and kept until 10 dpf. B. Percent survival of larvae exposed to SCFAs at 3 mM (SCFA3), 6 mM (SCFA6) and 9 mM (SCFA9), from 3 to 10 dpf. Log-rank (Mantel-Cox), ****p < 0.0001 compared to control. N = 90–130. C. Percent survival of larvae exposed to TNBS 50 μg/mL (T50) from 7 to 10 dpf and treated with SCFA 3, 6 and 9 mM from 3 to 10 dpf. Log-rank (Mantel-Cox), ****p < 0.0001 compared to control. N = 100–136. D. Percent survival of larvae exposed to TNBS 50 μg/mL (T50), 75 μg/mL (T75) and 100 μg/mL (T100) from 7 to 10 dpf and treated with SCFA 3 mM from 3 to 10 dpf. Log-rank (Mantel-Cox), ****p < 0.0001 compared to control. N = 70–80.