Fig. 4.

SCFAs decrease TNBS-induced inflammation. A. Representative images of the intestinal area (yellow dotted line) of Tg(lysC:DsRed2)^nz50 transgenic larvae after 72h (10 dpf) of 50 μg/mL (T50) and 75 μg/mL (T75) TNBS exposure and treated with 3 mM of SCFAs (SCFA3). Rostral to the left and dorsal up. Scale bar 200 μm. B. Graphical representation of the number of lysC: DsRed + cells (neutrophils) in the intestine of 10 dpf larvae. One-way ANOVA with Tukey's multiple comparisons. ****p < 0.0001. N = 7–26. C-E. Relative expression of il1b(C), il6(D), and tnfa(E), in whole larvae after 24, 48 and 72h of TNBS 50 μg/mL exposure and treated with SCFA 3 mM. Two-way ANOVA with Tukey's multiple comparisons. *p < 0.05; **p < 0.01; ***p < 0.001; ***p < 0.0001. N = 4–7. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)