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. 2021 Aug 31;2:142–154. doi: 10.1016/j.crimmu.2021.08.003

Table 1.

Bacteria phylum-specific primers.

Phylum Primer Sequences Reference
Total Bacteria (16S) Forward
Reverse
TGGCTCAGGACGAACGCTGGCGGC
CCTACTGCTGCCTCCCGTAGGAGT
Gómez-Hurtado et al. (2011)
Fusobacteria Forward
Reverse
CGAGGAACCTTACCAGCGTT
ATCTCACGACACGAGCTGAC
This study
Firmicutes Forward
Reverse
CAGCAGTAGGGAATCTTC
ACCTACGTATTACCGCGG
Pfeiffer et al. (2014)
Bacteroidetes Forward
Reverse
CRAACAGGATTAGATACCCT
GGTAAGGTTCCTCGCGTAT
Bacchetti De Gregoris et al. (2011)
Alphaproteobacteria Forward
Reverse
CIAGTGTAGAGGTGAAATT
CCCCGTCAATTCCTTTGAGTT
Bacchetti De Gregoris et al. (2011)
Betaproteobacteria Forward
Reverse
CGAAAAACCTTACCTACC
GTATGACGTGTGAAGCC
Pfeiffer et al. (2014)
Gammaproteobacteria Forward
Reverse
CCATGCCGCGTGTGTGAA
ACTCCCCAGGCGGTCAACTTA
Mühling et al. (2008)
Actinobacteria Forward
Reverse
GAGACTGCCGGGGTCAACT
TCTGCGATTACTAGCGAC
Pfeiffer et al. (2014)