Fig. 1.

Gating Strategy for Cytokine producing T cells: Whole blood samples from healthy controls (n = 5) were stimulated with PMA + ION, labelled with CD3 PerCP, lysed with 2.0 ml of 1 x FACS lysing solution and fixed with BFA before the labelling with Isotype Control (PE) and various cytokine antibodies (PE). The Flow cytometer dot plots illustrate the side scatter plot versus CD3-PerCP (A) with R1 gate for CD3⁺ lymphocytes (Total T cells), the Isotype Control plot for setting the gates (B), INF-γ producing cells (CD3+IFN-γ+ cells) (C), IL-10 producing T cells (CD3+IL-10+ cells) (D) and TNF-α producing T cells (CD3+TNF-α+ cells) (E).