Fig. 2.

Gating Strategy for Cytokine producing monocytes: Whole blood samples from healthy controls (n = 5) were stimulated with LPS, labelled with CD14 APC, lysed with 2.0 ml of 1 x FACS lysing solution and fixed with BFA before the labelling with Isotype Control (PE) and various cytokine antibodies (PE). Flow cytometer dot plots illustrating the side scatter plot versus CD14 (A) with R1 gate for CD14⁺ cells (monocytes), the Isotype Control plot for setting the gates (B), IL-6 producing monocytes (CD14+IL-6+ cells) (C) and TNF-α producing monocytes (CD14+TNF-α+ cells) (D).