Fig. 5.

Percentage of TNF-α and IL-6 producing monocytes at different stages of stimulation. Whole blood samples from healthy controls (n = 5) were stimulated with LPS for a duration of 4 h. At different time points (15 min, 30 min, 1 h, 1.5 h, 2 h, 2.5 h, 3 h, 3.5 h and 4 h) an aliquot of the stimulated blood was collected, labelled with CD14 APC, lysed with 2.0 ml of 1 x FACS lysing solution and fixed with BFA before the labelling with Isotype Control (PE) and either IL-6 PE or TNF-α PE and analysis by Flow Cytometry.