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. 2021 Oct 28;2:184–193. doi: 10.1016/j.crimmu.2021.10.002

Fig. 6.

Fig. 6

Percentage of T cells producing IL-2, IFN-γ and TNF-α at different time points following stimulation with PMA and Ionomycin. Whole blood samples from healthy controls (n = 5) were stimulated with PMA + ION. At different time points (from 15 min to 8 h) an aliquot of the stimulated blood was collected and labelled with CD3-PerCP, lysed with 2.0 ml of 1 x FACS lysing solution and fixed with BFA before the labelling with Isotype Control PE and IL-2 PE, INF-γ PE and TNF-α PE. The samples were then analysed by flow cytometry.