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. Author manuscript; available in PMC: 2022 Apr 26.
Published in final edited form as: Clin Chem. 2014 Mar 24;60(6):886–895. doi: 10.1373/clinchem.2013.219378

Fig. 1. Illustration of the Alu-HIV PCR strategy, showing primer positions of the Alu and HIV primers that exponentially amplify integrated HIV DNA (left).

Fig. 1.

The HIV-only PCR (right) linearly amplifies integrated and unintegrated HIV DNA. Both PCRs are run with multiple replicates. In the second quantitative PCR with nested primers and probes, for the LTR region, Cq values are obtained for each replicate reaction. The 95% CI is calculated from the HIV-only PCR (black bar delineated with the spheres). Cq values from the Alu-HIV PCR lower than this CI (dotted line) are considered positive replicates. ΔRn, the relative normalized fluorescence emission of each well.