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. Author manuscript; available in PMC: 2022 Apr 26.
Published in final edited form as: Clin Chem. 2014 Mar 24;60(6):886–895. doi: 10.1373/clinchem.2013.219378

Fig. 2. Serial dilutions of the integration standard assessed by repetitive-sampling Alu-HIV PCR and Poisson statistics.

Fig. 2.

(A) and (B), Linear relation between the expected integrated copies and the measured amount on 7500 cells (2 μg/mL) (A) or 37 500 cells (10 μg/mL) (B) per 50 μL replicate PCR. (C), The relation between expected and measured amounts is linear as long as <80% of PCR replicates are positive. This estimate becomes unpredictable at higher percentages of positive reactions. The values of the Poisson distribution are corrected (10-fold) on the basis of the integration standard calibrator.