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. 2020 Jun 27;1:23–37. doi: 10.1016/j.crimmu.2020.06.001

Fig. 5.

Fig. 5

Representative control-corrected SPR sensorgrams corresponding to the interactions of various TZM glycoforms with captured FcγRs. Sensorgrams corresponding to the interactions of fucosylated TZM (left column, TZM) or afucosylated TZM (right column, TZM RMD) with E5-tagged FcγRs previously captured on a K5 surface (≈30 RUs of FcγRIIIaF158 (a,b); ≈20 RUs of FcγRIIIaV158 (c,d); ≈20 RUs of FcγRIIa (e,f); ≈25 RUs of FcγRIIb (g,h) and ≈45 RUs of FcγRI (i,j)). Each data set corresponds to the triplicate injection of a TZM glycoform at 7 concentrations (between 40-4000 ​nM for fucosylated TZM-FcγRIIIaF158; 5–600 ​nM for afucosylated TZM-FcγRIIIaF158; 20-2000 ​nM for fucosylated TZM-FcγRIIIaV158; 3–300 ​nM for afucosylated TZM-FcγRIIIaV158; 100-8000 ​nM for FcγRIIa and FcγRIIb; 0.5–100 ​nM for FcγRI). (a-h) The steady state analysis of the sensorgrams is shown in the insets with the vertical line marking the respective calculated KD values. (i, j) The thin black solid lines correspond to the global fit using a kinetic model assuming the existence of two distinct populations of captured FcγRI.