REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
CD3 antibody	BD Biosciences	Cat#: 555275, RRID:AB_395699
CD11c antibody	BD Biosciences	Cat#: 550261, RRID:AB_398460
CD19 antibody	BD Biosciences	Cat#: 557399, RRID:AB_396682
Mouse Anti-NK-1.1	BD Biosciences	Cat#: 551114, RRID:AB_394052
CD62L antibody	BD Biosciences	Cat#: 560513, RRID:AB_10611578
I-A/I-E antibody	BD Biosciences	Cat#:562367, RRID:AB_11152078
Rat Anti- mouse CD44 antibody	BD Biosciences	Cat#: 559250, RRID:AB_398661
Mouse H-2K[b] antibody	BD Biosciences	Cat#: 562942, RRID:AB_2737908
Anti-Mouse CD80 antibody	BD Biosciences	Cat#: 553769, RRID:AB_395039
PE Rat Anti-Mouse CD86 antibody	BD Biosciences	Cat#: 553692, RRID:AB_394994
Goat Anti-Mouse IgG HRP antibody	R&D systems	Cat#: HAF007, RRID:AB_357234
InVivoMAb anti-mouse PD-1 (CD279)	Bio X cell	Cat#: BE0146, RRID:AB_10949053
Chemicals, peptides, and recombinant proteins
Clodronate liposomes and control liposomes	Liposoma	CSKU: CP-005-005
Recombinant Murine GM-CSF	PeproTech	Cat#: 315-03
Albumin from chicken egg white	Sigma-Aldrich	Cat#: A5503
Lipopolysaccharides from Escherichia coli O111:B4	Sigma-Aldrich	Cat#: L2630-10MG
Chlorophenicol red-β-D-galactopyranoside (CPRG)	Sigma-Aldrich	REF#: 10884308001
DNase type IV	Sigma-Aldrich	Cat#: D5025
Collagenase IV	Sigma-Aldrich	Cat#: C4-BIOC
Collagenase D	Sigma-Aldrich	Cat#: COLLD-RO
Cholic acid	Sigma-Aldrich	Cat#: C-1254
Tetramethylbenzidine Liquid Substrate System for ELISA	Sigma-Aldrich	Cat#: T0440-1L
MG132	Tocris Bioscience	Cat#: 1748/5
Lactacystin	Tocris Bioscience	Cat#: 2267
DQ™ ovalbumin	ThermoFisher Scientific	Cat#: D12053
Ovalbumin, Alexa Fluor™ 647 Conjugate	ThermoFisher Scientific	Cat#: O34784
AddaS03™ (AS03 -like squalene-based adjuvant)	InvivoGen	Cat#: vac-as03-10
AddaVax™ (Squalene-oil-in-water)	InvivoGen	Cat#: vac-adx-10
SIINFEKL peptide	GenScript	Lot#: U4778GB210-1/PE6339
Bovine Serum Albumin (BSA)	Bio Basic Canada Inc.	CAS#: 9048-46-8
β-Mercaptoethanol	Gibco	Cat#: 21985023
Sodium Pyruvate	Multicell	Cat#: 600-110-EL
Fetal Bovin Serum (FBS)	Multicell	Cat#: 090150
Phosphate Buffered Saline (PBS)	Multicell	Cat#: 311-010-CL
RPMI 1640	Multicell	Cat#: 350-000-CL
Penicillin/Streptomycin	Multicell	Cat#: 450-201-EL
L-glutamine	Multicell	Cat#: 609-065-EL
HEPES	Multicell	Cat#: 330-050-EL
MEM essential amino acid	Multicell	Cat#: 321-011-EL
DMEM	Multicell	Cat#: 319-005-CL
Bradford reagent	Bio-RAD	Cat#: 500-0006
Red blood cell lysis buffer	BioLegend	Cat#: 420301
Stop solution	R&D systems	Cat#: DY008
Coomasie Blue R-250	aMReSCO	Cat#: M128-25G
Tween20™	aMReSCO	Cat#: 0777-1L
Skim milk	Selection	Lot#: 231-03
Critical commercial assays
RNeasy Mini Kit (50)	Qiagen	Cat#: 74104
Mouse IFN-gamma DuoSet ELISA	R&D systems	Cat#: DY485-05
Mouse IL-2 DuoSet ELISA	R&D systems	Cat#: DY402-05
EasySep™ Mouse CD8a Positive Selection Kit II	Stemcell Technologies	Cat#:18953 and 18753
EasySep™ FITC for CD4 isolation	Stemcell Technologies	Cat#: 18558
Materials
G26 needle	Terumo	Cat#: NN2613R
0.45uM filter	UltiDent Scientific	Cat#: 229751
70uM cell strainer	ThermoFisher Scientific	Cat#: 22-363-548
Nunc MaxiSorpTM plates	ThermoFisher Scientific	Lot#: 1311523
96 wells culture plate	Sarstedt AG&CO KG	REF#: 82.1582.100
24 wells plate	Sarstedt AG&CO KG	REF#: 83.3922.300
Experimental models: Cell lines
Mouse: E.G7-OVA [derivative of EL4]	ATCC	ATCC Cat# CRL-2113, RRID: CVCL_3505
Mouse: EL4	ATCC	Cat# TIB-39, RRID: CVCL0255
Mouse: B3Z	Gift from Dr. Etienne Gagnon	N/A
Mouse: DC2.4	Sigma	SCC142
Experimental models: Organisms/strains
Mouse: BALB/cAnCrl	Charles River	Strain code: 028
Mouse: C57BL/6NCrl	Charles River	Strain code: 027
Mouse: OT-I (C57BL/6-Tg(TcraTcrb)1100Mjb/J)	The Jackson Laboratory	Strain #003831
Mouse: OT-II (B6.Cg-Tg(TcraTcrb)425Cbn/J)	The Jackson Laboratory	Strain #004194
Recombinant DNA
eGFP-hGal3	Addgene	Plasmid #73080
Software and algorithms
FlowJo v10	FlowJo™	https://www.flowjo.com/solutions/flowjo/downloads
Prism-GraphPad	GraphPad Software	https://www.graphpad.com/scientific-software/prism/
MATLAB	MathWorks	https://www.mathworks.com/products/matlab.html
SWISS-MODEL	SWISS-MODEL	https://swissmodel.expasy.org/
RCSP PDB	RCSP PDB	https://www.rcsb.org/3d-view/