Figure 1.

Analysis of circulating CD4⁺ and CD8⁺ T cell subsets from upper extremity recipients over time

(A) Skin biopsies and peripheral blood were collected over time (pre-transplantation, 24 h, 1 week, and 3, 6, and 12 months after transplantation) from upper extremity transplant recipients. PBMCs were isolated for posterior flow cytometry analysis. The cartoon was created with BioRender.

(B and C) Mean percentages of blood CD4⁺ (B) and CD8⁺ (C) naive cells (CCR7⁺CD45RA⁺), central memory T cells (TCM cells; CCR7⁺CD45RA⁻), effector memory T cells (TEM cells; CCR7⁻CD45RA⁻), and effector memory RA cells (TEMRA cells; CCR7⁻CD45RA⁺) before transplantation and 6 and 12 months after transplantation, represented as pie charts. Data are from all three individuals.

(D) Representative contour plots of gating of T helper (Th) 1 (CD4⁺CXCR3⁺CCR6⁻), Th2 (CD4⁺CXCR3⁻CCR6⁻), and Th17 (CD4⁺CXCR3⁻CCR6⁺) cells.

(E) Mean percentages of Th1, Th2, and Th17 cells from all three individuals over time.

(F–H) Mean percentages of circulating Th17 cells before transplantation and 12 months after transplantation. Statistic by paired t test, ∗p < 0.05. (G and H) Representative contour plots and mean percentages of (G) regulatory T (Treg) cells (CD4⁺CD25⁺CD127^−/low) and (H) T follicular helper (Tfh) cells (CD4⁺CXCR5⁺PD-1⁺) from all three individuals over time.

Graphs are displayed as mean ± SD at each time point examined.

See also Figure 1, Figure 2, Figure 3, Figure 4, Figure 5, Figure 6and S2.