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. Author manuscript; available in PMC: 2022 Apr 26.
Published in final edited form as: Neurobiol Dis. 2020 Sep 12;146:105078. doi: 10.1016/j.nbd.2020.105078

Fig. 3. optoTDP43 forms blue light dependent insoluble species that persist following removal of blue light.

Fig. 3.

A. Representative blots of soluble/insoluble (S/I) fractionation of gmr-GAL4;UAS-optoTDP43 adult heads staining for TDP-43 and tubulin as loading control with and without light stimulation at 48 hours, 96 hours, and 192 hours of light. B. Quantification of dose dependent formation of insoluble species from representative blots shown in panel A. n=3 blots per condition **p<.01, *p<.05. error bars +/− SD. 1-way ANOVA with test for multiple comparisons between each condition. C. Representative western blots of soluble/insoluble fractionation of gmr-GAL4;UAS-optoTDP43 adult heads staining for TDP-43 after 72 hours of light stimulation, 72 hours of light followed by 72 hours of dark, and 72 hours followed by 144 hours of dark. D. Quantification of relative amounts of insoluble/soluble optoTDP43 after 72 hours of light stimulation and then 72 and 144 hours after removal of blue light. n = 3 blots per condition **p<.01, *p<.05 error bars +/− SD. 1-way ANOVA with Tukey’s test for multiple comparisons.