TABLE 5.
EFFECT OF REMOVAL OF THE ZONA PELLUCIDA ON CELL FATE AT BLASTOCYST OF INDIVIDUAL BLASTOMERES LABELED WITH DII-CM AT THE 2-CELL STAGE
| Type of ovulation | Treatment* | Embryo development | Localization of Dil-CM positive cells | ||||
|---|---|---|---|---|---|---|---|
| Total embryos | Number of blastocysts | Total | Random | Embryonic | Abembryonic | ||
| S | Control | 8 | 8 (100 ± 0)a | ||||
| S | ZP− | 11 | 10 (92 ± 17)a | ||||
| S | DiI | 16 | 13 (81 ± 13)b | 13 | 3 (21 ± 14)a | 5 (40 ± 13)a | 5 (40 ± 13)a |
| S | DiI/ZP− | 32 | 27 (84 ± 6)b | 26 | 18 (70 ± 8)b | 5 (19 ± 5)b | 3 (11 ± 7)b |
Data are from five experiments. Numbers in parentheses are percentages ± S.D.
a,b
Values within columns with different superscripts are significantly (P < 0.05) different.
*
2-cell embryos were cultured either without any treatment (Control) or after removal of ZP (ZP−). Some 2-cell stage blastomeres were tagged with CM-DiI without removing the ZP (DiI). Others were tagged with DiI and the ZP removed (DiI/ZP−).