Fig. 4.

LINC00022 promoted migration and invasion in CRC cells. After HCT116, DLD1, and CaCo-2 cells were infected with LINC00022 low expression lentivirus or high expression lentivirus, the migration (Scale bar = 200 μm) and invasion (Scale bar = 100 μm) in HCT116, DLD1, and CaCo-2 cells were measured using Wound-healing assay and Transwell assay (a and b); Relative protein expression levels of MMP2 and MMP9 were assessed using Western blot (c). β-actin served as internal control. Data were presented as mean ± standard deviation (SD). N = 3. **P < 0.01 and ***P < 0.001 vs Lv-anti-NC group or Lv-NC group. MMP2, matrix metalloproteinase 2; MMP9, matrix metalloproteinase 9