Fig. 6.

LINC00022 upregulated FOXF1 expression through sponging miR-375-3p. (a) The binding sites between LINC00022 and miR-375-3p were predicted by LncBase v.2 and verified by dual luciferase assay. *P < 0.05, **P < 0.01 and ***P < 0.001 vs wt-LINC00022 + miR-375-3p mimics group. After HCT116, DLD1, and CaCo-2 cells were infected with LINC00022 low expression lentivirus or high expression lentivirus, the relative mRNA levels of miR-375-3p and FOXF1 were assessed by qRT-PCR (b); **P < 0.01, and ***P < 0.001 vs Lv-anti-NC group or Lv-NC group. Relative protein levels of FOXF1, STAT3, and p-STAT3 were calculated using Western blot (c). β-actin served as the internal control. (d) The binding sites between miR-375-3p and FOXF1 were predicted by TargetScanHuman 7.2 and verified by dual luciferase assay. **P < 0.01 vs wt-FOXF1 + miR-375-3p mimics group. Data were presented as mean ± standard deviation (SD). N = 3. FOXF1, Forkhead Box F1; STAT3, signal transducer and activator of transcription 3, and qRT-PCR, quantitative Real-time PCR