Fig. 7.

miR-375-3p knockdown reversed the effects of LINC00022 down-regulation on cell function in CRC. After LINC00022-silenced HCT116 cells were infected with Lv-anti-miR-375-3p mimic or Lv-anti-miR-NC, the viability of HCT116 cells was measured by CCK-8 assay (a); The migration (Scale bar = 200 μm) and invasion (Scale bar = 100 μm) of HCT116 cells were measured using Wound-healing assay and Transwell assay, respectively (b and c); Relative protein levels of FOXF1, c-Myc, pro caspase 3, cleaved caspase 3, MMP2, and VEGFA were assessed by Western blot (d). β-actin served as internal control. The content of VEGFA in cell supernatant was measured by detection kit (e). Data were presented as mean ± standard deviation (SD). N = 3. **P < 0.01 vs Lv-anti-LINC00022 + Lv-anti-miR-NC group. NC, negative control; FOXF1, Forkhead Box F1; MMP2, matrix metalloproteinase 2; VEGFA, vascular endothelial growth factor-A